Accurate estimation of kidney function is crucial to the detection, evaluation, and treatment of chronic kidney disease (CKD). The traditional methods are inaccurate or invasive. Here, we used DTI-MRI to evaluate kidney function with disease progression in nephrotoxic serum- induced CKD model (NTN). We detect changes associated with renal inflammation and fibrosis prior to functional decline. Moreover, AD and ADC of the cortex-outer medulla border were highly correlated with histologic evidence of fibrosis. These results suggest that DTI-MRI can detect early stages of CKD non-invasively in-vivo and can be used for fibrosis mapping in a variety of renal diseases.