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Abstract #2485

Mapping spatially heterogeneous single nephron function in the kidney using MRI.

Edwin J. Baldelomar1, Jennifer Charlton2, and Kevin M Bennett3
1Radiology, Washington University in St. Louis School of Medicine, St. Louis, MO, United States, 2Pediatric Nephrology, University of Virginia, Charlottesville, VA, United States, 3Radiology, Washington University in St. Louis School of Medicine, Saint Louis, MO, United States

Synopsis

Nephrons are the functional units of the kidney, coordinated with surrounding nephrons through the vasculature to filter blood plasma. We used in vitro CFE-MRI combined with Gd-DTPA contrast enhanced MRI to dynamically map single nephron physiology throughout the isolated, perfused rat kidney. We were able to map the heterogeneity and spatial variability of macromolecular binding and single nephron filtration. MRI appears to measure similar dynamics in single nephron filtration as intra-vital optical microscopy, with the advantage of measuring all nephrons in the kidney.

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