In this study was shown the possibility to perform compartment-specific metabolic investigations of living fibroblasts in real-time using a bioreactor system within an NMR spectrometer. Cell volume and the ratio cells to extracellular medium within the sensitive region of NMR coil were evaluated for quantification purposes. The needed sensitivity to detect metabolite changes in the extracellular footprint at different flow rates was shown. The capability of diffusion technique to distinguish between compartment-specific contributions was revealed, and to detect changes in compartment-specific metabolite pools under standard or selective culture conditions, and upon inhibitor challenges.
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