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Abstract #2752

Measuring lactate metabolism in vivo in at physiological level using [1-13C]lactate hyperpolarised with an endogenous labile radical precursor

Adam Philip Gaunt1, Jennifer Lewis1, Friederike Hesse1, Irene Marco-Rius1, Kevin Brindle1, and Arnaud Comment1,2
1University of Cambridge, Cambridge, United Kingdom, 2General Electric Healthcare, Chalfont St Giles, United Kingdom


A hyperpolarised [1-13C]lactate solution prepared by dynamic nuclear polarization at 7T and 1.4K using photo-irradiated alpha-ketoglutarate as the polarizing agent was injected at low dose in healthy rodents to allow measuring in vivo metabolism in the rodent brain and liver at physiological concentration. As a result of the large 13C polarisation, it was possible to detect metabolic products of [1-13C]lactate in vivo, including [1-13C]pyruvate and 13C-bicarbonate. The observed pyruvate-to-lactate ratios were commensurate with in vitro values previously reported in the literature. The purely endogenous composition of the solution offers the possibility of performing similar measurements in humans.

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