We present data from a pilot study of an Octopus rubescens arm demonstrating it is possibly to identify the complex structural arrangement of its musculature using MRI and DTI tractography. All major muscle groups of the octopus arm are able to be identified and extracted using DTI tractography. Overall, this pilot study establishes cephalopod arms as an appealing testbed for the development of muscle MRI segmentation and tractography algorithms.
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