This study investigated the orientation dependence of myelin water fraction (MWF) as evaluated with a 3D multiple-spin echo sequence (MSE). MSE and diffusion tensor scans were acquired at 7-T from two excised rat brains at three different orientations with respect to the main magnetic field. Region of interest analysis of the corpus callosum and the internal capsule revealed that there is no clear orientation dependency for MWF in these white matter tracts. This result is also evidenced by the minimal orientational modulation of the T2 spectra for both ROIs across both brains.
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