Amide proton transfer weighted (APTw) imaging is a novel molecular imaging technique to acquire the proton exchanged signals from the amide in proteins or peptides to water. Previous studies discovered lateralization in the brain structure and function, including the subcortical gray matter nucleus. However, it is not clear whether APTw also has lateral advantage. In this study, we applied the automatic brain segmentation method to quantify the APTw signal values of subcortical gray matter nucleus in young healthy subjects. The increased APTw signals were present in the right side of the subcortical gray matter nucleus，which may suppose related with right-handedness.