Punctate white matter lesion (PWML) is the most common injury in neonates. Due to newborns are a vulnerable population, the limited imaging protocols are not sufficient to fully understand the injury. Le Bihan et.al recently proposed a virtual MR elastography (vMRE) method based on multiple b-values diffusion sequences, which is attractive for evaluation of brain development and injury. This study aims to quantitatively assess the stiffness of PWML using vMRE. Compared with white matter regions, a significant increased virtual sheer stiffness is observed in lesions, and it may be a feasible clinical evaluation of the pathophysiological state of brain tissue.