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Abstract #3270

A Radial Flow Cell Perfusion System for Hyperpolarized 13C NMR Metabolic Studies at Low Oxygen Levels

Anthony Mancuso1, Mehrdad Pourfathi2, Ryan M. Kiefer2, Michael C Noji2, Sarmad Siddiqui2, Enri Profka2, Stephen J Kadlecek2, Rahim Rizi2, and Terence PF Gade1,2
1Cancer Biology, University of Pennsylvania, Philadelphia, PA, United States, 2Radiology, University of Pennsylvania, Philadelphia, PA, United States


We have developed new methods for studying cultured cancer cell metabolism with hyperpolarized 13C magnetic resonance spectroscopy (HP 13C MRS) at low oxygen concentrations. Cells grown on the surfaces of microcarriers inside the spectrometer were perfused radially (rather than axially) in a modified NMR tube at controlled oxygen levels. Computational and experimental results demonstrated that cell mass oxygen profiles with radial flow were much more uniform than with conventional axial flow. The metabolism of HP [1-13C]pyruvate was markedly different between the two flow configurations, demonstrating the importance of avoiding large oxygen gradients in cell perfusion experiments.

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