Quantitative cerebrovascular reactivity MRI in mice using acetazolamide challenge
Zhiliang Wei1,2, Yuguo Li1,2, Xirui Hou3, Zheng Han1,2, Jiadi Xu1,2, Michael T. McMahon1,2, Wenzhen Duan4,5, Guanshu Liu1,2, and Hanzhang Lu1,2,3
1Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, MD, United States, 2F. M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Research Institute, Baltimore, MD, United States, 3Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, United States, 4Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, United States, 5The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, United States
Cerebrovascular reactivity (CVR), which denotes brain’s vasodilatory capacity, is broadly utilized in cerebrovascular and neurodegenerative diseases. However, the most popular hypercapnia method in human studies is unsuitable for small animals due to the difficulty in measuring end-tidal CO2. Here, we took a different approach using a pharmacological vasodilatory stimulus, acetazolamide. Plasma level of acetazolamide and vascular responses were quantified by high-performance liquid chromatography and perfusion MRI, respectively. Evidences of feasibility, safety, temporal characteristics, and dose-dependence have been demonstrated. This new CVR technique may open several avenues for preclinical research on cerebrovascular diseases and therapeutic testing in different animal models.
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