Abstract #3269

Quantitative cerebrovascular reactivity MRI in mice using acetazolamide challenge

Zhiliang Wei^1,2, Yuguo Li^1,2, Xirui Hou³, Zheng Han^1,2, Jiadi Xu^1,2, Michael T. McMahon^1,2, Wenzhen Duan^4,5, Guanshu Liu^1,2, and Hanzhang Lu^1,2,3

¹Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, MD, United States, ²F. M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Research Institute, Baltimore, MD, United States, ³Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, United States, ⁴Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, United States, ⁵The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, United States

Synopsis

Cerebrovascular reactivity (CVR), which denotes brain’s vasodilatory capacity, is broadly utilized in cerebrovascular and neurodegenerative diseases. However, the most popular hypercapnia method in human studies is unsuitable for small animals due to the difficulty in measuring end-tidal CO2. Here, we took a different approach using a pharmacological vasodilatory stimulus, acetazolamide. Plasma level of acetazolamide and vascular responses were quantified by high-performance liquid chromatography and perfusion MRI, respectively. Evidences of feasibility, safety, temporal characteristics, and dose-dependence have been demonstrated. This new CVR technique may open several avenues for preclinical research on cerebrovascular diseases and therapeutic testing in different animal models.

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