T1ρ and T2 contrasts provide complementary information on tissue properties. Therefore, their transient recovery after exercise to pre-exercise equilibria is potentially a functional biomarker for muscle disorder diagnosis and treatment evaluation. In this pilot study, we implemented and tested a high-temporal-resolution dynamic MRI sequence for simultaneous T1ρ and T2 mapping immediately after exercise. T1ρ and T2 contrasts presented unique transient recovery time courses in different skeletal muscles. Dynamic information derived from our imaging approach could potentially lead to greater sensitivity and specificity in evaluating muscle conditions compared with traditional methods with either T1ρ or T2 contrast alone.