Diffusion tensor imaging (DTI) has emerged as biomarker to characterize the ligamentization process of the anterior cruciate ligament (ACL), following surgical reconstruction. One major limitation to this approach is the necessity for pre-injury baseline measurements to be used as a frame of reference, in order to inform clinical decision making with respect to recovery of the graft, and return-to-activity (RTA). Here, we explore the linear relationship for anisotropy between knees bilaterally, in healthy participants, to provide a methodological framework that allows for the clinical integration of DTI to monitor recovery of the tendon autograft, following surgical reconstruction, and inform RTA.
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