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Abstract #3434

Fluorine-19 MRI of Stem Cell-Derived Alveolar-Like Macrophages Tagged with Perfluoropolyether

Janny Yeyoung Kim1,2, Michael Litvack1, Daniel Li1, Brandon Zanette1, Martin Post1, and Giles Santyr1,2
1Translational Medicine, The Hospital for Sick Children, Toronto, ON, Canada, 2Medical Biophysics, University of Toronto, Toronto, ON, Canada


When stem cell-derived alveolar-like macrophages (ALMs) are labeled with perfluoropolyether (PFPE), they can potentially be monitored and tracked longitudinally in vivo using 19F MRI. In this work, different concentrations of PFPE and 5 million PFPE-labeled ALMs were imaged in vitro using 19F MRI at 3T. The results demonstrated the feasibility of detecting a small number of PFPE-labeled ALMs. This work will provide guidance for the upcoming in vivo instillation experiments in rat lungs. Longitudinal in vivo studies will further aid ALMs to be clinically translated as a stem cell therapy to treat chronic lung diseases.

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