Quantitative Susceptibility Mapping of the Ex Vivo Human Brainstem with Luxol Fast Blue Staining Correlation
Priya S Balasubramanian1,2, Lingfei Guo2,3, Weiyuan Huang2,4, Ilhami Kovanlikaya2, Alisa Ramineni5, Nector Garcia5, Benjamin Liechty5, Jonathan Dyke2, Henning Voss2, Eric Aronowitz2, Pascal Spincemaille2, David Pisapia5, and Yi Wang2
1Electrical and Computer Engineering, CORNELL UNIVERSITY, New York City, NY, United States, 2Department of Radiology, Weill Cornell Medicine, New York City, NY, United States, 3Department of Radiology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, Jinan, Shandong, China, 4Department of Radiology, Hainan General Hospital, Hainan Hospital Affiliated to Hainan Medical College, Hainan, China, 5Department of Pathology and Laboratory Medicine, Weill Cornell Medicine and New York Presbyterian Hospital, New York City, NY, United States
This study utilized sub-millimeter 3T gradient echo MRI of the brainstem ex vivo to obtain a magnetic susceptibility map (QSM). ROIs of both deep brain nuclei and myelinated fiber tracts were chosen and quantified from QSM obtained from MRI field. The ex vivo specimens were then stained with Luxol Fast Blue and the resultant absorbance of the ROIs were compared to QSM. A strong linear correlation was observed (R2 = 0.897, p = 0.0041).
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