qBOLD permits noninvasive measurements of the two critical determinants of the BOLD signal, i.e., deoxygenated-blood-volume (DBV) and venous-oxygen-saturation-level (Yv), and along with CBF imaging, the cerebral metabolic rate of oxygen (CMRO2). Two major challenges in qBOLD are 1) separation of heme-originated R2′ from other signal sources, and 2) subsequent extraction of DBV and Yv. We had previously addressed these issues by developing a prior-guided qBOLD method. Here, we aimed to evaluate the method's utility in 3D CMRO2 mapping. Results suggest feasibility of the new qBOLD method as a practical means for measuring neurometabolic parameters over an extended brain coverage.