Tomoelastography, a newly emerging imaging modality, is a multi-frequency magnetic resonance elastography technique using noise-robust data post-processing. The aim of our study was to investigate the assessment of stiffness and fluidity of pancreas in healthy volunteers with tomoelastography. Tomoelastography derived pancreatic stiffness and fluidity were near-perfect reproducible in healthy volunteers. There was no stiff or fluidic difference among different groups of sex, age, BMI or pancreatic anatomical region. Tomoelastography can provide stable and promising stiffness and fluidity measurements throughout the pancreas. Our results provide data that will enable pancreatic studies of tomoelastography as a potential clinical tool in future.