The aim of our study was to 1) provide spectrum of values of multi-echo mDixon MRI-PDFF, FFMRS, and FFs measured by two different histopathologic methods (pathologist and automatic fat vacuole segmentation), and 2) to evaluate the correlation among them. The values of MRI-PDFF and FFMRS were significantly higher than FFAI and significantly lower than FFpathologist. Except for one case (97.9%,46/47), FFMRS always showed higher value than MRI-PDFF and the average difference was 2.06 %. MRS and MRI-PDFF showed strong correlation with each other and with each histopathologic method. MRI-PDFF or MRS could be used as an alternative non-invasive reference standard.