This prospective study evaluated the performance of 3D magnetic resonance elastography imaging for predicting microvascular invasion(MVI) of T1 staging renal clear cell carcinoma. 80 consecutive patients were enrolled. The mean MRE stiffness value has significant difference between the tumor with MVI (5.4±0.6 Kpa) or without MVI (4.1±0.3 Kpa) (p<0.05). The sensitivity, specificity, positive predictive value, negative predictive value, AUC of stiffness value to predict MVI were 100%, 75%, 63%, 96%, 0.87, respectively. 3D MRE imaging has promising diagnostic performance for T1 staging renal clear cell carcinoma MVI.