The fast clearance of small molecular probes limits their applicability as responsive agent and prevents their use in longitudinal study. We show here the design and implementation of a tripod-type responsive agent for Zn2+ mapping that assembled to 100nm nanostructure at physiological temperature. The designed fluorinated probe showed capabilities to monitor Zn2+ when combining CEST MRI and 19F-MRI. Upon its intracranial injection the synthetic probe assembled to large entities in the tissue that prevent its clearance from the injection site. This property allows to map its 19F-signal for more than 20 hours and will enable longitudinal studies in the future.