Abstract #4647

Glycolytic Flux Alterations Following Radiotherapy in Cancer and Immune Cells: Hyperpolarized Carbon-13 Magnetic Resonance Imaging Study

Ying-Chieh Lai^1,2, Ching-Yi Hsieh^2,3,4, Kuan-Ying Lu^1,2, Albert P Chen⁵, Shu-Hang Ng^1,4, Fang-Hsin Chen^4,6, and Gigin Lin^1,2,3,4

¹Department of Medical Imaging and Intervention, Chang Gung Memorial Hospital at Linkou and Chang Gung University, Taoyuan, Taiwan, Taoyuan, Taiwan, ²Clinical Metabolomics Core Laboratory, Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan, Taoyuan, Taiwan, ³Medical Imaging Research Center, Institute for Radiological Research, Chang Gung University, Taoyuan, Taiwan, Taoyuan, Taiwan, ⁴Department of Medical Imaging and Radiological Sciences, Chang Gung University, Taoyuan, Taiwan, Taoyuan, Taiwan, ⁵GE Healthcare, Toronto, ON, Canada, Toronto, ON, Canada, ⁶Radiation Biology Research Center, Institute for Radiological Research, Chang Gung University, Taoyuan, Taiwan, Taoyuan, Taiwan

Synopsis

Alterations in metabolism following radiotherapy affect therapeutic efficacy, although the mechanism underlying such alterations is unclear. The present study demonstrates the potential for monitoring early metabolic alterations in both cancer and immune cells following radiation treatment by using hyperpolarized ¹³C-MRI. We demonstrated that cancer cells (human FaDu squamous carcinoma) and immune cells (HMC3 microglial cells and THP-1 monocytes) had distinct metabolic responses to ionizing radiation. Western blot analysis confirmed the similar trends in LDHA and LDHB expression levels. We furthered the knowledge of metabolic alterations resulting from not only cancer cells but also immune cells in the tumor microenvironment.

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