Abstract #4647
Glycolytic Flux Alterations Following Radiotherapy in Cancer and Immune Cells: Hyperpolarized Carbon-13 Magnetic Resonance Imaging Study
Ying-Chieh Lai1,2, Ching-Yi Hsieh2,3,4, Kuan-Ying Lu1,2, Albert P Chen5, Shu-Hang Ng1,4, Fang-Hsin Chen4,6, and Gigin Lin1,2,3,4
1Department of Medical Imaging and Intervention, Chang Gung Memorial Hospital at Linkou and Chang Gung University, Taoyuan, Taiwan, Taoyuan, Taiwan, 2Clinical Metabolomics Core Laboratory, Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan, Taoyuan, Taiwan, 3Medical Imaging Research Center, Institute for Radiological Research, Chang Gung University, Taoyuan, Taiwan, Taoyuan, Taiwan, 4Department of Medical Imaging and Radiological Sciences, Chang Gung University, Taoyuan, Taiwan, Taoyuan, Taiwan, 5GE Healthcare, Toronto, ON, Canada, Toronto, ON, Canada, 6Radiation Biology Research Center, Institute for Radiological Research, Chang Gung University, Taoyuan, Taiwan, Taoyuan, Taiwan
Synopsis
Alterations in metabolism following radiotherapy affect therapeutic efficacy, although the mechanism underlying such alterations is unclear. The present study demonstrates the potential for monitoring early metabolic alterations in both cancer and immune cells following radiation treatment by using hyperpolarized 13C-MRI. We demonstrated that cancer cells (human FaDu squamous carcinoma) and immune cells (HMC3 microglial cells and THP-1 monocytes) had distinct metabolic responses to ionizing radiation. Western blot analysis confirmed the similar trends in LDHA and LDHB expression levels. We furthered the knowledge of metabolic alterations resulting from not only cancer cells but also immune cells in the tumor microenvironment.
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