Conventional MRI is limited in reflecting tumor genetic status. This study used the parameters derived from histogram analysis of apparent diffusion coefficient(ADC) maps in glioma lesion to predict the MGMT and P53 gene status. We found the ADC histogram derived parameters are significantly higher in MGHT-methylated and P53 wild group. ROC analysis revealed these parameters have high sensitivity to differentiate the MGHT-methylated/-unmethylated or P53 wild/ mutant gliomas. These findings suggest ADC histogram could be a good candidate for predicting MGMT promoter methylation and P53 gene status in gliomas.