This study compared the image quality of MRCP before and after contrast agent injection on a 3T MRI scanner using the same respiratory-triggered 3D-SPACE sequence. Our results showed that enhancement of contrast-to-noise ratio (CNR) and background suppression will make the contour of pancreaticobiliary system more clear, which indirectly improves the imaging effect of MRCP. We performed MRCP scanning during the delayed waiting period for enhanced scans, which shortens the overall scan time for such patients. In summary, respiratory-triggered 3D-SPACE MRCP imaging with enhanced contrast injection significantly improved the imaging quality of the pancreaticobiliary duct and shorten the overall acquisition time. It is worthy of promotion and application in clinical practice.