Keywords: Cancer, Cancer, Clear Cell Renal Cell Carcinoma
Motivation: To develop targeted therapies for Clear Cell Renal Cell Carcinoma (ccRCC).
Goal(s): Investigation of metabolic activity in von Hippel-Lindau (VHL) patient derived renal tumor cells using NMR based stable isotope tracing to develop targeted tumor therapies.
Approach: Cultured VHL patient derived renal tumor cells (UOK270 and UOK 366) were harvested and extracted upon 24 h of [U-13C] Glucose tracing and analyzed using NMR based stable isotope Resolved Metabolomics (SIRM).
Results: After 24 h of [U-13C] Glucose tracing, we determined the relative levels of 13C incorporation in Glycolysis and (Tri Carboxylic Acid) TCA cycle intermediates in UOK 270 and UOK366 using NMR based SIRM.
Impact: This is the first-time investigation of metabolic activity in VHL patient derived renal tumor cells generated in house (UOK270 and UOK366) impacting on ccRCC treatment development.
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