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Abstract #1864

Dual use of nitroxyl radicals as a polarizing agent in hyperpolarization 13C-MRS and as a redox probe in EPR spectroscopy to study acute kidney injury

Abdelazim Elsayed Elhelaly1,2, Manal Habaka3, Fuminori Hyodo4,5, Yoshifumi Noda6, Hiroki Kato7, and Masayuki Matsuo6
1Department of Radiology, Frontier Science for imaging, Gifu University, Gifu, Japan, 2Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt, 3Animal Health Research Institute, Zagazig Provincial Laboratory, Zagazig, Egypt, 4Center for One Medicine Innovative Translational Research (COMIT), Institute for Advanced Study, Gifu University, Gifu, Japan, 5Department of Radiology, Gifu University, Gifu, Japan, 6Department of Radiology, School of Medicine, Gifu University, Gifu, Japan, 7Gifu University, Gifu, Japan

Synopsis

Keywords: Hyperpolarized MR (Non-Gas), Metabolism, Electron Paramagnetic Resonance, Ex vivo Molecular Imaging

Motivation: Trityl radicals are the main polarizing agents used for hyperpolarization. However parallel monitoring of metabolic and redox conditions is an unmet need.

Goal(s): To use nitroxides as a polarizing agent and as a redox probe simultaneously for the same sample.

Approach: Four nitroxyl probes were tested. HyperSense DNP polarizer was used for 13C-pyruvate hyperpolarization.13C-MRS and EPR measurements were performed using same samples.

Results: CmP showed the highest hyperpolarization signal. A significant reduction in 13C lactate production and EPR decay rates of CmP during disease progression were confirmed.

Impact: We described successful application of nitroxyl radicals for simultaneous assessment of energy metabolism and redox status.

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Abstract

Keywords