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Abstract #1944

Within-session test-retest reproducibility and reliability of auditory cortex GABA+ in individuals with tinnitus using MEGA-sLASER at 3T

Jonathan M. Wai1,2, Kelley Swanberg3, Alex Grassetti2, Martin Gajdošík4, Diana Martinez1,2, and Christoph Juchem5
1Department of Psychiatry, Columbia University Irving Medical Center, New York, NY, United States, 2New York State Psychiatric Institute, New York, NY, United States, 3Department of Experimental Medicine, Lund University, Lund, Sweden, 4Synex Medical, Boston, MA, United States, 5Department of Biomedical Engineering, Columbia University, New York, NY, United States

Synopsis

Keywords: Head & Neck/ENT, Spectroscopy, Precision and accuracy, brain, tinnitus

Motivation: The measurement of GABA+ using in vivo proton magnetic resonance spectroscopy requires adequate reproducibility and reliability for its use in clinical research and as a potential biomarker.

Goal(s): We aimed to determine the within-session test-retest reproducibility and reliability of GABA+ in the auditory cortex of individuals with tinnitus.

Approach: A MEGA-sLASER sequence for GABA+ measurement using two consecutive within-session blocks was performed on 38 participants over 2 scanning days.

Results: GABA+ quantification in the auditory cortex demonstrated similar reproducibility and reliability when using either a total creatine (tCr) or water reference (GABA+/tCr: CV=5.6%, ICC=0.69, 95% CI [0.52-0.80]; GABA+/water: CV=5.6%; ICC=0.73, 95% CI [0.58-0.83]).

Impact: Auditory cortex GABA+ quantification with MEGA-sLASER in a clinical population with GABA+ abnormalities in the region of interest can be achieved with reproducibility and reliability that is comparable to what has been reported in other brain regions of healthy controls.

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Keywords