Abstract #2300

Stem Cell-Derived Extracellular Vesicles Restore Sodium & Energetic Homeostasis in Ischemic Stroke as Quantified by Longitudinal MRI/S at 21.1 T

Jamini Bhagu^1,2 and Samuel Colles Grant^1,2

¹Center for Interdisciplinary Magnetic Resonance, National High Magnetic Field Laboratory, Tallahassee, FL, United States, ²Chemical & Biomedical Engineering, Florida State University, Tallahassee, FL, United States

Synopsis

Keywords: Stroke, High-Field MRI, Spectroscopy, Metabolites, Stem Cell Therapy, Neuroinflammation, Preclinical

Motivation: A leading cause of death and disability, stroke is treated foremost by restoring blood flow, but follow-up therapies that focus on ischemic tissue recovery are critical as is the evaluation of therapeutic efficacy.

Goal(s): This work administers extracellular vesicles (EV) derived from human mesenchymal cells (hMSC) to salvage tissue while monitoring recovery and metabolic changes longitudinally using ultra-high field MRI/S.

Approach: T₂-W MRI,²³Na-CSI, and ¹H-MRS were used to quantify lesion reduction, sodium homeostasis and energetic remodeling.

Results: Upon EV treatment, sodium (and to a lesser extent proton) lesions were reduced by day 3, while lactate, creatine and NAA were stabilized compared to control.

Impact: Combined sodium MRI and proton MRS provide a more sensitive and early quantitative metric to evaluate the efficacy of stem cell-derived therapy following ischemia and longitudinal metabolic, ionic and functional recovery. Such methods can evaluate other treatments against different pathologies.

How to access this content:

For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.

After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.

After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.

Click here for more information on becoming a member.