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Abstract #2615

Comparing R2* and QSM at 9.4T for the ability to detect increased deoxyhemoglobin (hypoxia) in the mouse brain

Ty Makarowski1,2,3, Hongfu Sun4, and Jeff F Dunn1,2,3
1Department of Radiology, University of Calgary, Calgary, AB, Canada, 2Department of Neuroscience, University of Calgary, Calgary, AB, Canada, 3Hotchkiss Brain Institute, University of Calgary, Calgary, AB, Canada, 4School of Information Technology and Electrical Engineering, University of Queensland, Queensland, Australia

Synopsis

Keywords: Susceptibility/QSM, Quantitative Imaging, Hypoxia, High-Field MRI

Motivation: Investigating R2* and QSM for deoxyhemoglobin detection is vital for diagnosing and understanding diseases where tissue oxygenation is frequently compromised.

Goal(s): To evaluate R2* against QSM for their ability to detect deoxyhemoglobin changes in a controlled hypoxic environment using a mouse model.

Approach: Employ a graded hypoxia protocol in naïve, female C57Bl/6 mice, capturing 3D MGE images at various oxygen levels (30%, 15% and 10%) to measure R2* and QSM responses.

Results: R2* demonstrated significant sensitivity to hypoxia in brain regions, particularly the hippocampus, unlike QSM, suggesting its potential as a superior hypoxia biomarker.

Impact: This study reveals R2* relaxometry's superior sensitivity to the detection of changes in deoxyhemoglobin over QSM, potentially improving early detection and monitoring of hypoxia-related diseases, such as Multiple Sclerosis, and informing future clinical imaging protocols.

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