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Abstract #3058

Fluorinated gelatin-based scaffolds traceable by 19F MRI

Vít Herynek1, Kristýna Kolouchová2, Jiří Karela1, Lana Van Damme2, Luděk Šefc1, and Sandra Van Vlierberghe2
1Center for Advanced Preclinical Imaging (CAPI), First Faculty of Medicine, Charles University, Prague, Czech Republic, 2Department of Organic and Macromolecular Chemistry, Ghent University, Ghent, Belgium

Synopsis

Keywords: Non-Proton, Preclinical, Gelatin scaffolds, 19F MRI/MRS

Motivation: Gelatin-based scaffolds may be seeded by cells or may serve as depots for drugs and ensure their slow release.

Goal(s): The goal of the study was to prepare scaffolds with tunable properties and monitor their stability in time in a mouse model in vivo.

Approach: The scaffolds implanted to mice were monitored by 1H/19F MRI, fluorine content was quantified by 19F MR spectroscopy.

Results: The scaffolds can be easily monitored by 19F MRI/MRS. The study confirmed that the design of the scaffolds can be fine-tuned for future applications, biodegradation rates may be set from several weeks up to one year.

Impact: Gelatin-based scaffolds pave the road towards qualitatively different drug applications and regenerative medicine. The study evaluated their applicability, traceability by 1H/19FMRI, and stability in vivo. Future work should investigate release speed of different compounds (drugs) both hydrophilic and hydrophobic.

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Keywords