Abstract #3195

Quantify sodium transmembrane transport in cells via relaxation exchange spectroscopy

Yu Yin¹, Juntao Xia^2,3, Yun Chen¹, Ruiliang Bai^4,5,6, and Xueqian Kong^1,2,3,4

¹Department of Chemistry, Zhejiang University, Hangzhou, China, ²Institute of Translational Medicine, Shanghai Jiao Tong University, Shanghai, China, ³School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai, China, ⁴Department of Physical Medicine and Rehabilitation，Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, China, ⁵Key Laboratory of Biomedical Engineering of Education Ministry, College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, China, ⁶Interdisciplinary Institute of Neuroscience and Technology, School of Medicine, Zhejiang University, Hangzhou, China

Synopsis

Keywords: Spectroscopy, Non-Proton, odium, transmembrane transport

Motivation: Transmembrane transport of sodium ions is directly related to the cell functions and metabolisms and could be an indicator of various diseases such as neurodevelopmental disorders, neuropathic pain, etc. There is a lack of non-invasive and clinically-adaptable techniques for quantifying the rate of transmembrane transport of sodium ions.

Goal(s): Determine the rate of sodium transmembrane transport using noninvasive nuclear magnetic resonance methods.

Approach: ²³Na relaxation exchange spectroscopy (REXSY) was applied to the cellular systems for quantitative analysis.

Results: The ²³Na REXSY method successfully determined the sub-second transmembrane exchange rate of sodium ions in yeast and HeLa cells.

Impact: Measuring transmembrane rate of sodium via NMR can assist the pathological studies of diseases related to malfunctions of sodium ion channels and/or sodium metabolism. ²³Na REXSY could be coupled to MR imaging to offer novel parameters for clinical diagnosis.

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