Keywords: fMRI Analysis, fMRI, preclinical fMRI, awake fMRI, multimodal imaging, whisker stimulation, whisker tracking
Motivation: Anesthesia causes alterations in brain functional data and internal brain states affect sensory perception.
Goal(s): We sought to investigate how the Blood Oxygen Level Dependence (BOLD) response from spontaneous and stimulated whisker motion in awake mice is related to cerebral arousal state monitored by acetylcholine.
Approach: Mice with implanted RF coils and fiber optic cable were simultaneously imaged and acetylcholine signals were monitored to measure correlation between BOLD and acetylcholine signals.
Results: We found stimulating the whiskers produced the expected contralateral BOLD response in awake animals but also an acetylcholine spike in the ipsilateral side indicating whole-brain dynamic activation occurs in awake mice.
Impact: This work shows feasibility of simultaneous high-resolution EPI-based functional imaging and fiber photometry of acetylcholine response to whisker stimulation in awake mice as a multimodal method for investigating brain state from external sensory stimulation using implantable RF coils.
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