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Abstract #4031

In Vivo Assessment of Cerebral β-Hydroxybutyrate Metabolism in APPNL-F/NL-F Mouse Model of Alzheimer's Disease using 2H MR Spectroscopy

Narayan Datt Soni1, Anshuman Swain1, Paul Jacobs1, Halvor Juul1, and Ravinder Reddy1
1Department of Radiology, University of Pennsylvania, Philadelphia, PA, United States

Synopsis

Keywords: Alzheimer's Disease, Deuterium, 2H-MRS, Brain, Alzheimer's disease, Metabolism

Motivation: Among all, glucose hypometabolism is a characteristic pathology in Alzheimer’s disease (AD), under such conditions β-Hydroxy butyrate (BHB) can serve as an alternate source of energy. Measurement of BHB metabolism using a sensitive method could serve as a method for early diagnosis of AD.

Goal(s): To monitor BHB metabolism in APPNL-F/NL-F mice.

Approach: Monitoring cerebral 2H2-Glx labeling in mice following subcutaneous administration of 2H4-BHB using pulse-acquired 2H-MRS.

Results: Although rate of cerebral 2H4-BHB uptake was similar in wild type (WT) and AD mice, moderately enhanced labeling of 2H2-Glx was observed in AD mice compared to WT indicates relatively efficient BHB metabolism in AD.

Impact: Although BHB metabolism has been monitored in human and rodent brains using PET and 13C-MRS, inherent limitations restrict their clinical translation. Using a safe and sensitive method like 2H-MRS could be helpful in studying BHB metabolism in various neurodegenerative diseases.

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