Keywords: Neurofluids, Brain, Glymphatic System
Motivation: This study investigates the glymphatic function in awake vs. anesthetized mice by means of diffusion-weighted (DW) MRI.
Goal(s): Application of developed ‘silent’ DW-MRI protocol is presented for comparison of awake and anesthetized mice under ketamine-xylazine (K/X), and isoflurane.
Approach: In total, n=17 C57BL/6 mice were habituated to awake MRI setup, and successfully underwent DW-MRI with monitored body temperature and respiratory rate.
Results: Results showed that brain water mobility in awake mice was consistent, while K/X and isoflurane affected both slow and fast MR diffusivity measures differently. Awake DW-MRI offers a promising alternative to MRI under anesthesia, promoting increased biological relevance and translational potential.
Impact: This research on glymphatic function using DW-MRI underscores the importance of non-invasive MRI in awake mice, emphasizing anesthesia's impact on physiological stability. It highlights the translational potential of awake DW-MRI and is of importance to neuroscientific and biomedical research communities.
How to access this content:
For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.
After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.
After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.
Keywords