Abstract #4445

Cell Surface Mannose Overexpression as New CEST MRI Biomarker for Glioblastoma Aggressiveness

Behnaz Ghaemi¹, Shreyas Kuddannaya¹, Hernando Lopez Bertoni², John Joseph Laterra², Guanshu Liu³, and Jeff W.M Bulte⁴

¹Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering, Johns Hopkins University, Baltimore, MD, United States, ²Department of Oncology, Department of Neurology, Johns Hopkins University, Baltimore, MD, United States, ³Russell H. Morgan Department of Radiology and Radiological Science, F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Inc., Johns Hopkins University, Baltimore, MD, United States, ⁴Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering, Department of Oncology, F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Inc., Johns Hopkins University, Baltimore, MD, United States

Synopsis

Keywords: CEST / APT / NOE, CEST & MT, Glioblastoma, Cancer stem cells, Label-free CEST

Motivation: Non-invasive grading of glioblastoma (GBM) aggressiveness is critical for choosing a proper treatment paradigm

Goal(s): To develop a label-free MRI technique that can probe the presence of cancer stem cells (CSCs) in GBM.

Approach: To detect high mannose N-linked glycans overexpressed on mesenchymal CSCs with mannose-weighted (MANw) CEST MRI.

Results: MANw CEST MRI was able to produce a distinct signal for highly aggressive GBM tumor spheres but not for low aggressive ones, which corresponded to the histoptahological presence or absence of mannose expression.

Impact: We present a simple, non-invasive diagnostic approach for assessment of glioblastoma aggressiveness, which can be immediately implemented as an add-on to current MRI protocols.

How to access this content:

For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.

After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.

After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.

Click here for more information on becoming a member.