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Abstract #4915

Adiabatic T1rho-mapping of subacute myocardial ischemia with ultra-short echo time 3-D imaging in ex vivo mice

Iida Räty1, Antti Paajanen2, Mikko Nissi2, Sanna Kettunen1, Anna-Kaisa Ruotsalainen1, Svetlana Laidinen1, Seppo Ylä-Herttuala1, and Elias Ylä-Herttuala1
1A.I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio, Finland, 2Department of Technical Physics, University of Eastern Finland, Kuopio, Finland

Synopsis

Keywords: Myocardium, Quantitative Imaging, Ex vivo applications, High field MRI, Small animals, Ischemia, Preclinical imaging

Motivation: Conventional CMR techniques have many limitations, such as slowness and inability to provide contrast-agent free 3-D assessments of myocardium in the cardiac diseases.

Goal(s): The aim of this study is to develop a faster, high-resolution 3-D CMR imaging method for quantitative imaging of the myocardium after myocardial infarction (MI).

Approach: Mice hearts were imaged ex vivo 7 days after the factitious MI by using an ultra-short echo time 3-D T technique (MB-SWIFT-CS).

Results: The T relaxation times were elevated in the infarct area. The findings were validated by 2-D CMR maps and histopathology to confirm ischemia, edema, and fibrosis in the myocardium.

Impact: This study presents a quantitative ultra short echo time 3-D CMR method for ex vivo assessment of myocardium after factitious myocardial infarction in mice. The method allowed rapid comprehensive 3-D myocardial evaluation by utilizing compressed sensing and relaxation time mapping.

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Keywords