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Abstract #0292

Investigating neuron-specific BOLD coupling via simultaneous two-photon microscopy imaging and rs-fMRI of the mouse brain at 16.4T

Yuncong Ma1, Guangle Zhang1, Wei Zhu1, Hannes M Wiesner1, Zongyue Cheng2, Chenmao Wang2, Kamil Ugurbil1, Xiao-Hong Zhu1, Meng Cui2, and Wei Chen1
1Radiology, University of Minnesota, Minneapolis, MN, United States, 2Biological Sciences, Purdue University, West Lafayette, IN, United States

Synopsis

Keywords: Mesoscale: columns and layers, Multimodal, fMRI Calcium

Motivation: Resting-state fMRI (rs-fMRI) based on BOLD contrast is powerful for mapping brain connectivity and function. However, the underlying neural vascular coupling is still elusive.

Goal(s): To understand the neuron-specific contributions to the rs-fMRI BOLD signal.

Approach: We simultaneously acquired rs-fMRI and calcium images using MRI-compatible two-photon microscopy imaging (TPMI) in mice brains at 16.4T; the signals from both imaging modalities were analyzed and correlated within the resting-state networks of interest.

Results: Neurons show distinct correlations to the BOLD signal, with clustered spatial distributions across cortical layers, and the neuron co-activation patterns (NCAP) show time-dependent activity to drive BOLD events.

Impact: We investigated neuron-specific BOLD coupling to be spatially distinct and time-dependent at resting state using simultaneous TPMI and rs-fMRI on mice at 16.4T. The findings provide new insights into how clustered neurons drive BOLD signal fluctuations in the resting brain.

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Keywords