Meeting Banner
Abstract #0300

Dynamic Hyperpolarized MRI for Imaging ¹³C-Ketoisocaproate Metabolism in an Alzheimer’s Disease model

Ingeborg Sæten Skre1, Ditte Bentsen Christensen1, Andrea Strange Fredriksen1, Magnus Karlsson1, Rie Beck Olin1, and Mathilde Hauge Lerche1
1Health technology, Technical University of Denmark (DTU), Kongens Lyngby, Denmark

Synopsis

Keywords: Hyperpolarized MR (Non-Gas), Metabolism, Brain, Carbon-13

Motivation: With hyperpolarized 13C-MRI, α-keto-[1-13C]isocaproate (KIC) conversion to leucine can be probed in vivo. While static imaging has shown potential, dynamic acquisitions may enhance metabolic investigations.

Goal(s): Our goal was to develop and validate a dynamic imaging strategy to assess cerebral KIC metabolism in vivo, using an animal model of Alzheimer’s Disease (AD).

Approach: Transgenic 5xFAD (n=3) and wild-type (n=3) mice received KIC injections. Dynamic imaging of KIC’s conversion to leucine was conducted using an IDEAL spiral CSI acquisition-method.

Results: The results demonstrated rapid leucine accumulation in the brain, with a significantly higher cerebral metabolic conversion to leucine in AD mice compared to controls.

Impact: We introduce a dynamic method to measure BCAT activity in vivo, with initial results indicating increased activity in a preclinical Alzheimer's model. By enabling real-time tracking of KIC metabolism, we provide a tool for investigating metabolic alterations in neurological disorders.

How to access this content:

For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.

After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.

After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.

Click here for more information on becoming a member.

Keywords