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Abstract #0565

CEST Z-spectra fitting via Bloch-McConnell equations for pH mapping: in vitro validation and application in healthy and pathological leg muscles

Valentin Henriet1, Pierre-Yves Baudin1, Constantin Slioussarenko1, Benjamin Marty1, Marc Lapert2, and Harmen Reyngoudt1
1Neuromuscular Investigation Center, NMR Laboratory, Institute of Myology, Paris, France, 2Siemens Healthcare SAS, Courbevoie, France

Synopsis

Keywords: Muscle, CEST & MT, pH, fitting, skeletal muscle, neuromuscular diseases

Motivation: Intramuscular pH is a promising biomarker for neuromuscular disease research which can be assessed with ¹H and ³¹P MRS, though these techniques have limitations, such as reduced spatial resolution.

Goal(s): Chemical Exchange Saturation Transfer (CEST) enables high-resolution pH mapping by measuring exchange rates between metabolites and free water.

Approach: In this study, we used a 3-pool Bloch-McConnell model to fit and extract the exchange rate between creatine and free water.

Results: Sensitivity was assessed in vitro within the physiological pH range and in vivo in leg muscles of healthy volunteers, at rest and post-exercise, and in a patient’s leg.

Impact: This work advances high-resolution pH mapping for neuromuscular disease assessment, offering non-invasive insights into muscle homeostasis. By enhancing sensitivity and spatial resolution in pH measurement, it could improve the monitoring of muscle disorders, benefiting clinical research.

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