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Abstract #0705

Diffusion MRI of organoids at 28.2T with 3T/m gradient strength

Chantal M.W. Tax1,2, Tatiana Nikolaeva3,4, Channa Jakobs5,6, Rico Singer7, Jeroen Pasterkamp5,6, and Julia Krug4
1Center for Image Sciences, University Medical Center Utrecht, Utrecht, Netherlands, 2CUBRIC, Cardiff University, Cardiff, United Kingdom, 3University Medical Center Utrecht, Utrecht, Netherlands, 4Laboratory of BioNanoTechnology, Wageningen University & Research, Wageningen, Netherlands, 5Dept. of Translational Neuroscience, University Medical Center Utrecht, Utrecht, Netherlands, 6Utrecht Brain Center, Utrecht University, Utrecht, Netherlands, 7ICube laboratory, University of Strasbourg, Strasbourg, France

Synopsis

Keywords: Simulation/Validation, High-Field MRI, Preclinical ultra-high field MRI

Motivation: Organoids are a promising alternative to current human and animal imaging platforms for validating and designing quantitative MRI measurements.

Goal(s): To develop diffusion MRI (dMRI) on living organoids at ultra-high field (28.2T).

Approach: A variety of diffusion measures were evaluated in cortical organoids using high-resolution dMRI at 28.2T, such as ADC, kurtosis, diffusion time-dependence and diffusion anisotropy. Longitudinal stability of organoids was evaluated. Finally, a Gd-based contrast agent was tested to accelerate measurement times.

Results: dMRI of living organoids is feasible at ultra-high-field with high spatial resolution, down to 20x20x20µm3. Various diffusion measures can be probed, such as kurtosis, anisotropy and diffusion time-dependence.

Impact: Ultra-high field MRI organoids can provide a complementary platform to human and animal imaging for validation and design of quantitative MRI. Furthermore, dMRI of organoids adds a complementary non-invasive organoid assessment method to optical and electron microscopy.

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Keywords