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Abstract #0717

Micron-resolution fiber mapping in brain histology independent of sample preparation

Marios Georgiadis1, Franca auf der Heiden2, Hamed Abbasi3,4, Loes Ettema3, Jeffrey Nirschl1, Hossein Moein Taghavi1, Moe Wakatsuki1, Andy Liu1, William Hai Dang Ho1, Mackenzie Carlson1, Michail Doukas4, Sjors A. Koppes4, Stijn Keereweer4, Raymond A. Sobel1, Kawin Setsompop1, Congyu Liao1, Katrin Amunts2,5, Markus Axer2,6, Michael Zeineh1, and Miriam Menzel2,3
1Stanford University School of Medicine, Stanford, CA, United States, 2Forschungszentrum Jülich GmbH, Julich, Germany, 3Delft University of Technology, Delft, Netherlands, 4University Medical Center Rotterdam, Rotterdam, Netherlands, 5University Düsseldorf, Düsseldorf, Germany, 6University of Wuppertal, Wuppertal, Germany

Synopsis

Keywords: Structural Connectivity, Validation, fiber orientations, scattered light imaging

Motivation: Mapping brain’s intricate fiber network is a key quest in neuroscience. However, diffusion MRI is expensive, slow, and probes thousands of fibers/voxel, while micron-resolution methods typically do not resolve crossings, probe limited filed-of-view, or need special sample preparations.

Goal(s): To map fibers in brain sections from any sample preparation with micron-resolution.

Approach: We use computational scattered light imaging (ComSLI) with a simple rotating light and camera setup on new or archived brain histology sections.

Results: We generate micron-resolution fiber maps from histology sections of healthy and diseased human and animal brains, prepared with different protocols, providing ground-truth information for diffusion MRI fiber mapping.

Impact: Using scattered light, we generate micrometer maps of brain fiber orientations in animal and human histology sections, and compare to diffusion MRI. This enables time- and cost-effective studies of brain micro-architecture on new and archived sections from any sample preparation.

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Keywords