Keywords: Small Animals, Neuro, Respiration;Inhibitory neurons;Pontine Reticular Nucleus;Opto-fMRI;
Motivation: While the role of inhibitory neurons in the pontine reticular nucleus caudalis (PnC) in respiratory control has been identified, its neural network and vascular responses remain unclear.
Goal(s): Thus, we aim to investigate the brain network underlying slow breathing induced by inhibitory neuron activity in PnC.
Approach: Using optogenetic fMRI, we targeted PnC inhibitory neurons in awake, self-breathing anesthetized and intubated anesthetized mice.
Results: Our findings revealed widespread BOLD responses in awake and self-breathing anesthetized conditions, and mapped respiratory-related brain networks under mechanical ventilation with intubation, highlighting the PnC’s role in respiratory regulation.
Impact: This study introduces a method to distinguish global vascular responses from neural-driven BOLD responses, enabling investigations into respiratory circuits and identifying previously unreported sites involved in respiratory control.
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