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Abstract #0837

Optogenetic fMRI of Pontine Reticular Nucleus for Mapping Respiratory Networks

Tong Jin1,2, Geun Ho Im1, Sang-Han Choi1, Thanh Tan Vo1,2, Sung Han3, and Seong-Gi Kim1
1Center for Neuroscience Imaging Research (CNIR), Institute for Basic Science (IBS), Suwon, Korea, Republic of, 2Department of Biomedical Engineering, Sungkyunkwan University, Suwon, Korea, Republic of, 3The Salk Institute for Biological Studies, San Diego, CA, United States

Synopsis

Keywords: Small Animals, Neuro, Respiration;Inhibitory neurons;Pontine Reticular Nucleus;Opto-fMRI;

Motivation: While the role of inhibitory neurons in the pontine reticular nucleus caudalis (PnC) in respiratory control has been identified, its neural network and vascular responses remain unclear.

Goal(s): Thus, we aim to investigate the brain network underlying slow breathing induced by inhibitory neuron activity in PnC.

Approach: Using optogenetic fMRI, we targeted PnC inhibitory neurons in awake, self-breathing anesthetized and intubated anesthetized mice.

Results: Our findings revealed widespread BOLD responses in awake and self-breathing anesthetized conditions, and mapped respiratory-related brain networks under mechanical ventilation with intubation, highlighting the PnC’s role in respiratory regulation.

Impact: This study introduces a method to distinguish global vascular responses from neural-driven BOLD responses, enabling investigations into respiratory circuits and identifying previously unreported sites involved in respiratory control.

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