Keywords: Probes & Targets, Cell Tracking & Reporter Genes, Deuterium, probe, target, cancer, biomarker
Motivation: In the era of gene transfer, there is a need for non-invasive quantitative mapping of genetically engineered reporters that are applicable for clinically-translatable imaging modalities.
Goal(s): To develop a quantitative 2H-MRI-based approach to map the expression of the HSV1-TK reporter gene, which is widely used for PET.
Approach: A highly deuterated synthetic analog of thymidine (d6-5MDHT) was synthesized and proposed as a 2H-MRI reporter probe for mapping the expression of the HSV1-TK reporter gene.
Results: We showed the ability to quantitatively map, in vivo, with 2H-MRI, the HSV1-TK_7B expression upon the accumulation of the administered d6-5MDHT only in tissues expressing the transgene.
Impact: The successful implementation of the proposed 2H-MRI reporter system will allow the longitudinal quantitative mapping of a reporter gene expression, elucidating the kinetics and efficiency of gene and cell therapies employed in basic research and the clinic.
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