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Abstract #1484

Impact of Tissue Sample Preparation Method on Myelin-Sensitive Quantitative MR Imaging and Histological Analysis

Amaya Murguia1, Andrea Jacobson2, Scott D. Swanson3, Ulrich Scheven4, Jon-Fredrik Nielsen2,3, Jeffrey A. Fessler1,2,3, and Navid Seraji-Bozorgzad5
1Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, MI, United States, 2Biomedical Engineering, University of Michigan, Ann Arbor, MI, United States, 3Radiology, University of Michigan, Ann Arbor, MI, United States, 4Mechanical Engineering, University of Michigan, Ann Arbor, MI, United States, 5Neurology, University of Michigan, Ann Arbor, MI, United States

Synopsis

Keywords: White Matter, White Matter

Motivation: Quantitative MRI (qMRI) parameter validation with histology is often done with ex vivo fixed tissue. Freezing is another preservation method, but the effects of freezing/thawing on qMRI parameters and their correlation with histology are unknown.

Goal(s): We investigated how freezing/thawing brain tissue affects myelin-sensitive qMRI properties and histology correlation.

Approach: We scanned the same fresh/thawed and fresh/fixed samples and compared qMRI parameters with luxol fast blue (LFB) results.

Results: qMRI values correlated well with LFB across conditions. Thawed and fixed tissues exhibited modest increases in qMRI parameters compared to fresh. Histology showed that samples did not lose tissue integrity from the freezing process.

Impact: Tissue freezing is a reasonable alternative preservation method to tissue fixation for use in qMRI analysis. Brain banks that store frozen tissue could use these samples for future qMRI and histological studies.

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Keywords