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Abstract #1530

Concurrent SORDINO fMRI and Calcium Imaging at Cellular Resolution Reveal Prefrontal Cortex Integration with Brain Networks

LiMing Hsu1, Martin MacKinnon2, Sheng Song2, Randy Nonneman2, Sung-Ho Lee3, Jose Rodríguez-Romaguera4, and Yen-Yu Ian Shih3
1Department of Radiology and BRIC, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States, 2Department of Neurology, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States, 3Department of Neurology and BRIC, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States, 4UNC Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States

Synopsis

Keywords: Multimodal, fMRI (resting state), small animals

Motivation: To investigate how regional neuronal activity from distinct cellular clusters are associated with fMRI-derived large-scale brain networks.

Goal(s): To develop a multimodal imaging platform for awake mice, bridging knowledge across measurement scales and modalities.

Approach: Using MR-compatible calcium imaging at cellular resolution to simultaneously measure fMRI network dynamics and cellular activity in awake mice.

Results: This platform reveals multiple network correlates with distinct cellular clusters within the mouse medial prefrontal cortex, suggesting spatial integration of these functionally segregated neurons in an evolutionarily conserved species.

Impact: We introduce a radically new approach combining brain-wide fMRI and cellular-level calcium imaging, enabling researchers to tackle brain network questions previously beyond reach.

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