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Abstract #1957

Spatial biomechanics quantification of the Tumoural MicroEnvironment of a HepG2 spheroids with Magnetic Resonance Elastography

Marguerite Ducamp1, Anne-Sophie Van Schelt2, Valerie Paradis3, Adel Hammoutene4, Lina Aguilera Munoz3, Aurelie Beaufrere3, Asma Boumaza5, Gabrielle Mangin5, Maddy Parsons1, and Ralph Sinkus2,6
1School of Biomedical Engineering and Imaging Sciences, King's College London, London, United Kingdom, 2King's College London, London, United Kingdom, 3Centre de Recherche sur l'Inflammation, INSERM U1149, Paris, France, 4Centre de Recherche sur l'Inflammation, INSERM 1149, Paris, France, 5INSERM UMRS1148 Laboratory for Vascular Translational Science, Paris, France, 6INSERM UMRS1148 Laboratory for Vascular Translational Science, University Paris, Paris, France

Synopsis

Keywords: Biology, Models, Methods, Cancer, organoids, high resolution, tumoural microenvironment

Motivation: The Tumoural-Micro-Environment (TME) is characterized by an increase in stiffness, hence the motivation to consider MR-Elastography for its characterization.

Goal(s): We present an experimental setup to locally characterize the biomechanics of cancer cells spheroid embedded in a collagen matrix using Magnetic Resonance Elastography (MRE).

Approach: Using MRE at 35 microns resolution, we can locally quantify the biomechanic of a spheroid embedded in a collagen matrix.

Results: Comparing the biomechanical properties of a tumour spheroid with an agar spheroid, we show that tumour spheroid modulates their microenvironment within a perimeter of 0.9mmx1.8mm.

Impact: Developing 3D culture model, starting from patient’s biopsy samples, that is predictive to their in vivo response to treatment, easily applicable could be relevant in the field of tailored medicine. This could spare the patient non-effective chemotherapy exposure or surgery.

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Keywords