Keywords: Biomarkers, Spectroscopy
Motivation: Standard MRS allows the detection of total choline but has insufficient resolution to separate choline from phosphocholine and glycerophosphocholine.
Goal(s): To develop 1H-14N 2D HSQC methods to allow separate detection of all 1H and 2H cholines.
Approach: 1H detection of choline methylene protons provides high detection sensitivity and chemical shift separation of choline types. 14N provides high sensitivity towards methyl group deuteration. All features can be combined in 2D HSQC through scalar coupling between 1H and 14N.
Results: 2D HSQC allows the detection of all six forms of protonated and deuterated choline forms as demonstrated on RG2 cells and rat brain extracts.
Impact: A single 1H-14N 2D HSQC scan can quantitatively map all forms of protonated and deuterated choline, phosphocholine and glycerophosphocholine, opening the possibility of comprehensive study of dynamic 2H-choline transport, turnover and metabolism.
How to access this content:
For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.
After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.
After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.
Keywords