Abstract #3535

Applying Phosphorous Cross-Polarization/Magnetization Transfer to Probe the Membrane/Water Interface in Myelin

Cariad-Arianna Knight¹, Alex Ensworth^1,2, Cornelia Laule^1,2,3,4, Alex L MacKay^1,3,5, and Carl A Michal¹

¹Physics and Astronomy, University of British Columbia (UBC), Vancouver, BC, Canada, ²International Collaboration on Repair Discoveries (ICORD), Vancouver, BC, Canada, ³Radiology, University of British Columbia, Vancouver, BC, Canada, ⁴Pathology & Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada, ⁵UBC MRI Research Centre, University of British Columbia, Vancouver, BC, Canada

Synopsis

Keywords: Novel Contrast Mechanisms, Contrast Mechanisms, Myelin, White Matter, Dipolar, T1D, Lipid Membrane, Interface

Motivation: Most current MRI methods to study myelin rely solely on ¹H nuclei. Because ¹H is ubiquitous, this limits the specificity and detail of the information these methods can provide.

Goal(s): To develop a suite of information-rich techniques, based on a new combination of ³¹P cross-polarization with magnetization transfer, that are specific to lipid membrane/water interfaces.

Approach: We developed a family of pulse sequences that transfer membrane ³¹P magnetization to water (¹H_aq) to make relaxation and indirect spectroscopy measurements.

Results: These new sequences provide ¹H_aq-detected wideline semi-solid ¹H (¹H_s) and ³¹P NMR spectra and relaxation data, specific to the membrane/water interface in myelin.

Impact: Detecting membrane-specific ³¹P and ¹H_s NMR signals through MRI-visible ¹H_aq could provide new and extremely specific MRI contrast mechanisms for myelin, providing superior sensitivity to membrane condition than existing proton-only metrics.

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