Abstract #3542

Myelin Water Fraction Orientation Dependence: An Ex-Vivo 9.4T Human Spinal Cord Study

Tigris Joseph^1,2, Kirsten Bale^2,3, Andrew Yung^2,3, Sarah Morris^1,2,3, Piotr Kozlowski^2,3, Pushwant Mattu⁴, Lisa Parker⁴, Kevin Dong², Femke Streijger², G. R. Wayne Moore^2,5, Adam Velenosi², Veronica Hirsch-Reinshagen², Brian Kwon², Irene Vavasour^2,3, and Cornelia Laule^1,2,3,5

¹Physics and Astronomy, University of British Columbia, Vancouver, BC, Canada, ²International Collaboration on Repair Discoveries, University of British Columbia, Vancouver, BC, Canada, ³Radiology, University of British Columbia, Vancouver, BC, Canada, ⁴Pathology, Vancouver General Hospital, Vancouver, BC, Canada, ⁵Pathology & Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada

Synopsis

Keywords: Relaxometry, White Matter, Myelin, Orientation Dependence, Spinal Cord, Human, Post Mortem, High Field, 9.4T, Myelin Water Fraction, T2 Relaxation

Motivation: Recent in-vivo human and ex-vivo animal tissue research suggests that the Myelin Water Fraction (MWF) may depend on the angle of fibers relative to the main magnetic field.

Goal(s): To investigate MWF orientation dependence in human spinal cord tissue.

Approach: 9.4T multi-echo T₂ relaxation data was collected from ex-vivo human spinal cord tissue rotated compared to B₀. MWF in white matter was compared between 9 different angles.

Results: MWF varied with orientation. The average COV of mean white matter (WM) MWF across 4 samples was 12.9%. Most samples had a consistent WM MWF vs. angle pattern.

Impact: We observed variations in myelin water fraction (MWF) at different cord orientations relative to the B₀ field, suggesting that MWF is dependent on fiber orientation. Further work should focus on methods to correct for orientation dependence when scanning in-vivo.

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