Abstract #3763

7T 3D-MR Spectroscopic Imaging of GSH and GPX4 Immunohistochemistry Staining Reveal Oxidative Stress Patterns in MS Lesions

Rebeka Rumbak^1,2, Assunta Dal-Bianco^2,3, Fabian Niess¹, Bernhard Strasser¹, Lukas Hingerl¹, Anita Kloss-Brandstätter⁴, Simon Hametner^3,5, Romana Höftberger^3,5, Thomas Berger^2,3, Günther Grabner⁶, Paulus Rommer^2,3, Wolfgang Bogner^1,7, and Eva Niess¹

¹Department of Biomedical Imaging and Image-guided Therapy, HFMR, Medical University of Vienna, Vienna, Austria, ²Department of Neurology, Medical University of Vienna, Vienna, Austria, ³Comprehensive Center for Clinical Neurosciences and Mental Health, Medical University of Vienna, Vienna, Austria, ⁴Department of Engineering & IT, Carinthia University of Applied Sciences, Villach, Austria, ⁵Division of Neuropathology and Neurochemistry, Department of Neurology, Medical University of Vienna, Vienna, Austria, ⁶Department of Medical Engineering, Carinthia University of Applied Sciences, Klagenfurt, Austria, ⁷Christian Doppler Laboratory for MR Imaging Biomarkers, Vienna, Austria

Synopsis

Keywords: Multiple Sclerosis, biomarkers, Oxidative Stress, Multiple Sclerosis, Immunohistochemistry, Spectroscopy

Motivation: GSH in MS can reveal oxidative stress areas where the body mounts defence, but the mechanism of GSH counteraction remains poorly understood due to limitations of standard MRS.

Goal(s): By mapping GSH distribution across lesion types, we aim to elucidate antioxidative responses in the MS brain, informing potential therapeutic targets.

Approach: We used 7T high-resolution echo-less 3D-MRSI to map GSH levels in 22 MS patients and validated findings with GPX4 immunohistochemistry post mortem.

Results: The GSH/tCr ratio was elevated in lesions, particularly paramagnetic rim lesions, compared to normal-appearing WM; GPX4 staining indicated higher antioxidative activity in lesions than in normal-appearing and healthy WM.

Impact: High-resolution 7T 3D-MRSI mapping of GSH reveals oxidative stress patterns across MS lesions, enhancing in vivo lesion monitoring and offering comprehensive insights that support clinical management and progression tracking in multiple sclerosis.

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