Abstract #3862

CEST MRI detection of Glycoengineered Extracellular Vesicles

Safiya Aafreen¹, Kris Dammen-Brower², Wenshen Wang^3,4, Sixuan Li⁵, Xiaoning Han⁶, Tza-Huei Wang⁵, Kevin J. Yarema², and Guanshu Liu^3,4

¹Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, United States, ²Depeartment of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, United States, ³Department of Radiology, Johns Hopkins University School of Medicine, Baltimore, MD, United States, ⁴F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, MD, United States, ⁵Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, United States, ⁶Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, United States

Synopsis

Keywords: Contrast Agents, Contrast Agents, extracellular vesicle, glyoengineering, theranostics

Motivation: Extracellular vesicles (EVs) are innovative carriers for drug and gene delivery, yet current imaging relies on fluorescent, metallic, or isotopic labels, complicating clinical translation. Metabolic glycoengineering (MGE) may enhance EV targeting naturally by incorporating non-natural sugars into EV surfaces.

Goal(s):

To leverage CEST MRI for non-labeled detection of MGE-EVs in vivo.
To examine MGE’s impact on EV properties and optimize non-natural glycan selection.

Approach: MGE incorporates non-natural monosaccharides into EV surfaces, creating a profile suitable for CEST MRI-based visualization.

Results: In vitro and in vivo studies validate MGE-EVs as innovative theranostic platforms, with inherent imaging properties advancing clinical potential.

Impact: Leveraging non-natural sugars as CEST MRI agents equips MGE-EVs with advanced MRI tracking capabilities. This innovation is poised to accelerate both preclinical development and clinical translation of more effective MGE-EV-based drug delivery vectors for a wide variety of diseases.

How to access this content:

For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.

After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.

After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.

Click here for more information on becoming a member.