Abstract #4055

Observation of central nervous system and body fluid viscosity of Trypoxulus dichotomus (Coleoptera) in the pupal stage using 9.4-T MRI.

Shoto Ikegami^1,2,3, Ren Harada⁴, Yutarou Kusaba², Arata Horie², Yoshiki Oda⁵, Kinuko Niihara⁶, Masafumi Yoshida^6,7, Takashi Inoue A.⁷, and Kagayaki Kuroda^1,2,4

¹Course of Science and Technology, Graduate School of Science and Technology, Tokai University, Hiratsuka, Kanagawa, Japan, ²Department of Human and Information Sciences, School of Information Science and Technology, Tokai University, Hiratsuka, Kanagawa, Japan, ³Research Fellow of Japan Society for the Promotion of Science, Chiyoda-ku, Tokyo, Japan, ⁴Course of Electrical and Electronic Engineering, Graduate School of Engineering, Tokai University, Hiratsuka, Kanagawa, Japan, ⁵Technical Joint Management Office, Tokai University, Hiratsuka, Kanagawa, Japan, ⁶Department of Natural Sciences, Faculty of Science and Engineering, Tokyo City University, Setagaya-ku, Tokyo, Japan, ⁷Division of Natural Sciences, Graduate School of Integrative Science and Engineering, Tokyo City University, Setagaya-ku, Tokyo, Japan

Synopsis

Keywords: Diffusion Analysis & Visualization, biology, models, methods, metamorphosis, insect, pupa

Motivation: To clarify the misters metamorphosis process of holometabolous insects, which might lead to innovative technology of regenerative medicine

Goal(s): Elucidation of the process focusing on the central nervous system, and fluid viscosity relation to protein composition in the body fluid.

Approach: Consecutive observation of the tissue change by DTI and T2 mapping, as well as protein analysis by absorbance measurement and electrophoresis, was performed in the pupa of Trypoxylus dichotomus.

Results: The nerve system remained unchanged during metamorphosis period, while unimodal T2 changed, which may have related to protein composition, peaking 3-4 days after pupation were recognized.

Impact: The present results show that the nerve fibers and ganglia remain, while other tissues are developed. These findings give new insight into the relationship between the central nerve system and metamorphosis in holometabolous insects.

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